Overnight Whole Plasmid Sequencing.
From $15 / sample.

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Plasmids

Amplicons

Microbes

Colonies

AAV

Microbes

  • Long-Read DNA Sequencing. Fully sequence your entire strand in one run. No primers. Get faster, more accurate results.
  • Easy Drop-Off. Drop off your sample at one of our 721 convenient locations. Or just mail it to us.
  • Overnight Results. Submit your sample in the afternoon and get results in your inbox next morning.
Arnold J Federico

Arnold J Federico

@ArnoldJFederico

X Logo

Shoutout to @plasmidsaurus for not only sequencing my 12 samples in less than a day but also being able to identify stuff like entire plasmid duplications!

11:46 PM · Jun 4, 2024

Prerna Singh

Prerna Singh

@singhcoli

X Logo

Put some plasmids in the dropbox at 3pm yesterday and got the results at 02:55am. Less than 12 hours for the full annotated sequence of 4 plasmids is quite mind boggling

10:25 AM · Jan 30, 2022

Phillip Kyriakakis

Phillip Kyriakakis

@breakliquid

X Logo

Sequencing a 165kbp BAC containing large repeats with @plasmidsaurus. I feel more confident than ever on what I have. What an amazing technology

2:25 AM · Apr 24, 2024

Sebastian S. Cocioba

Sebastian S. Cocioba

@ATinyGreenCell

X Logo

I routinely sequence plasmids over 15kbp and pay a flat $15 with @plasmidsaurus for whole plasmid sequencing. Results next day!

8:24 PM · Aug 2, 2024

Anthony Berndt

Anthony Berndt

@anthony_berndt

X Logo

The time, money, and energy @plasmidsaurus saves shaves somewhere between 2 and 5 days off of every one of our design-build-test cycles.

7:55 AM · Jul 25, 2024

Arnold J Federico

Arnold J Federico

@ArnoldJFederico

X Logo

Shoutout to @plasmidsaurus for not only sequencing my 12 samples in less than a day but also being able to identify stuff like entire plasmid duplications!

11:46 PM · Jun 4, 2024

Prerna Singh

Prerna Singh

@singhcoli

X Logo

Put some plasmids in the dropbox at 3pm yesterday and got the results at 02:55am. Less than 12 hours for the full annotated sequence of 4 plasmids is quite mind boggling

10:25 AM · Jan 30, 2022

Phillip Kyriakakis

Phillip Kyriakakis

@breakliquid

X Logo

Sequencing a 165kbp BAC containing large repeats with @plasmidsaurus. I feel more confident than ever on what I have. What an amazing technology

2:25 AM · Apr 24, 2024

Sebastian S. Cocioba

Sebastian S. Cocioba

@ATinyGreenCell

X Logo

I routinely sequence plasmids over 15kbp and pay a flat $15 with @plasmidsaurus for whole plasmid sequencing. Results next day!

8:24 PM · Aug 2, 2024

Anthony Berndt

Anthony Berndt

@anthony_berndt

X Logo

The time, money, and energy @plasmidsaurus saves shaves somewhere between 2 and 5 days off of every one of our design-build-test cycles.

7:55 AM · Jul 25, 2024

Arnold J Federico

Arnold J Federico

@ArnoldJFederico

X Logo

Shoutout to @plasmidsaurus for not only sequencing my 12 samples in less than a day but also being able to identify stuff like entire plasmid duplications!

11:46 PM · Jun 4, 2024

Prerna Singh

Prerna Singh

@singhcoli

X Logo

Put some plasmids in the dropbox at 3pm yesterday and got the results at 02:55am. Less than 12 hours for the full annotated sequence of 4 plasmids is quite mind boggling

10:25 AM · Jan 30, 2022

Phillip Kyriakakis

Phillip Kyriakakis

@breakliquid

X Logo

Sequencing a 165kbp BAC containing large repeats with @plasmidsaurus. I feel more confident than ever on what I have. What an amazing technology

2:25 AM · Apr 24, 2024

Sebastian S. Cocioba

Sebastian S. Cocioba

@ATinyGreenCell

X Logo

I routinely sequence plasmids over 15kbp and pay a flat $15 with @plasmidsaurus for whole plasmid sequencing. Results next day!

8:24 PM · Aug 2, 2024

Anthony Berndt

Anthony Berndt

@anthony_berndt

X Logo

The time, money, and energy @plasmidsaurus saves shaves somewhere between 2 and 5 days off of every one of our design-build-test cycles.

7:55 AM · Jul 25, 2024

How it works

1. Place order

Choose your sample type and size, choose your payment method and place the order.

3. Overnight Results 

Results waiting for you in your inbox in the morning. Includes sequence files (.FASTA, .GBK, .FASTQ), plasmid map, and purity histogram.

2. Drop Off Sample 

Place sample at one of our over 700 locations. Or print a shipping label and drop in the mail.

Register Now

Sequence everything

Whole Plasmid Sequencing

Reveal structural and purity issues not detectable with Sanger sequencing.

Sequence everything

Fully sequence your entire plasmid, including insert and backbone, using the latest long-read Oxford Nanopore technology. Results include:

  • Sequence files - Get a polished consensus sequence of your plasmid. FASTA, .GBK, .ab1.
  • Plasmid map - See a graphical representation of your DNA sequence, including genes & promoters.
  • Read length histogram - View the read length distribution of your sample’s raw reads.
Sequence everything

Linear/PCR Sequencing

Get full-length coverage of PCR products. No primers.

Sequence everything

Quickly confirm clonal amplicons, and get deep sequencing for mixed populations. No primers, even for long amplicons. Results include:

  • Sequence files - View your sample as a polished consensus sequence. FASTA, .GBK, .ab1.
  • Plasmid map - Get a visual diagram of your DNA sequence. Genes, promoters, restrictions, & more.
  • Read length histogram - View the read length and number of your raw reads.
Sequence everything

ZeroPrep Sequencing

Sequence colonies directly. No mini-prep needed.

Sequence everything

Just package your colony/culture and send it to us. No need to isolate and purify the plasmid DNA. We’ll handle prep for you. Results include:

  • Sequence files - Get a polished consensus sequence of your sample. FASTA, .GBK, .ab1.
  • Plasmid map - See a graphical representation of your DNA sequence, including genes & promoters.
  • Read length histogram - View the read length distribution of your sample’s raw reads.
Sequence everything

Bacterial genome sequencing

Get whole-genome sequencing for clonal populations of bacteria.

Sequence everything

Sequence the entire genome of microbial organisms in one run. Fully annotated genome assembly with raw sequencing data. 1-day turnaround. Results include:

  • Sequence files - Get a polished consensus sequence of your genome. FASTA and FASTQ.
  • GenBank files - Get a polished & annotated consensus sequence. Delivered in .gbff.
  • Analytical report - A report of the key metrics for the assembly. N50, total number of contigs, & more.
Sequence everything

AAV Genome Sequencing

Get full-length sequencing of whole AAV genomes.

Sequence everything

Fully sequence AAV genomes, using purified, intact AAV viral capsids as input. Single-stranded and self-complementary configurations. Results include:

  • Sequence files - Get a polished consensus sequence of your sample. FASTA, .GBK, .ab1.
  • Read length histogram - View the read length distribution of your sample’s raw reads.
  • Isoform quantifications - See the percentage of each AAV genome variant from the total reads.
Sequence everything

Custom sequencing

Long-read sequencing for any project. Delivered in just 3-5 days.

Sequence everything

Get full-length sequencing for any double-stranded linear or circular DNA, up to 300 kb in length. Results include:

  • Raw .fastq - Unprocessed reads, including nucleotide sequences and quality scores.
Sequence everything

Full sequence. Overnight.
No primers.

Register Now

Unmatched Speed

Drop off in the afternoon and get results by the next morning.

Register Now

Simple Process

No extra prep. Drop off or mail. Real-time updates.

High Accuracy

Capture the full sequence. Genes, regulatory elements, mutations, etc.

Next-Gen Innovation

Exclusive access to the latest Oxford Nanopore tech.

Identify sample purity issues.
See what Sanger is missing.

A histogram with one dominant peak typically indicates a clean prep with a single plasmid.

A histogram with multiple peaks indicates unexpected products, deletions, recombinations, or concameters.

Order Now

Join Over 70,000 Scientists

Gordon Wellman

Gordon Wellman

PhD,
Uri Alon lab, Weizmann Institute

We have used Plasmidsaurus extensively in the past year or so to verify plasmid identities and to gain insight into the E. coli population we designed. We also used the service to follow competition and evolution experiments of differentiating E. coli, and to find structural variants in mutant “stem cells”.

Katie Galloway

Katie Galloway

Professor,
MIT

Before whole-plasmid sequencing, we knew our plasmids had issues but we couldn’t see them. It has allowed us to identify issue with vectors and avoid costly integration of these vectors into experiments and cloning workflows.

Arjun Raj

Arjun Raj

Professor,
University of Pennsylvania

We now have the confidence to know exactly what we are working with.

Anthony Berndt

Anthony Berndt

Senior Scientist,
Insmed Inc

If it weren't for your company, we'd easily be 6-9 months behind where we are now. That's at least half a year shaved off an already skunk-works mostly-vibes and longshot driven R&D program.

Cholpisit Ice Kiattisewee

Cholpisit Ice Kiattisewee

PhD Student,
University of Washington

Plasmidsaurus helps identify small changes in the plasmid backbone that was overlooked in the conventional method.

Gerry Smith

Gerry Smith

Professor,
Fred Hutchinson Cancer Center

Our lab has had excellent service from Plasmidsaurus sequencing bacterial and phage DNA we went them. When a run did not succeed, they readily, and freely, redid it and often got solid sequences. They made raw read data available for us to examine. All of this for an amazingly low price. And quick!

Rochelle Aw

Rochelle Aw

Academic Staff Researcher,
Stanford

Plasmidsaurus has become a vital tool for me in terms of completely upending the way that I approach cloning. Whenever I pull a plasmid from glycerol stocks, I always check that it is what we think it is, and this can be a game changer rather than sinking days or weeks into the wrong thing.

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